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Pcr2.1 topo vector

SpletpCR2.1-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®II-TOPO® but lacking an SP6 … SpletVector selection is performed by our team of specialists, and is dictated by the size of the insert and the complexity of the synthesis approach adopted. For a full listing of the standard cloning vectors we offer please contact our technical support team at [email protected]. Blue Heron Biotech 2. Custom Vectors

Addgene: pCR2.1_HBB_N

SpletThe recombinant plasmid pCR 2.1-TOPO/phoP was then introduced into the competent E. coli DH cella and single recombinant colonies were selected. The presence of insert DNA fragment was... http://www.protocol-online.org/biology-forums/posts/31897.html shanghai vice mayor zhang wei https://idreamcafe.com

Topo Ta Cloning Vector Pcr2 1 Thermo Fisher Bioz

Splet15. nov. 2007 · The TOPO TA vectors have an topisomerase attached so all you do is incubate your insert and the vector with a salt solution. Using the topoisomerase enzyme as opposed to DNA ligase is meant to be quicker and more efficient. I use TOPO TA pCR2.1 vector, which uses the blue white screening system - so you do need x-gal. It works really … SpletpCRII-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 … SpletTOPO® TA Cloning® Kit . Five-minute cloning of Taq polymerase-amplified PCR products . Catalog numbers (pCR ™ 2.1-TOPO ® vector) K4500-01, K4500-40, K4500-J10, K4510 … shanghai victoria kindergarten

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning

Category:Plasmids 101: TOPO Cloning - Addgene

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Pcr2.1 topo vector

InvitrogenTOPO TA Cloning Kit, with pCR2.1-TOPO, One Shot …

SpletInvitrogen TOPO PCR cloning technology was developed to help improve cloning efficiency, simplify protocol setup, and accommodate a wide range of PCR insert sizes. TOPO … SpletThermo Fisher pcr2 1 topo t a cloning shuttle vector Pcr2 1 Topo T A Cloning Shuttle Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

Pcr2.1 topo vector

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SpletDescription It uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. 3'-T overhangs for direct ligation of Taq -amplified PCR products Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. SpletPcr2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol …

Splet10. jul. 2013 · The plasmid backbone is from pCR2.1-Topo (Invitrogen) and contains an ampicillin resistance gene, a kanamycin resistance gene, a pUC ori, a M13 reverse primer and a T7 promoter sequencing primer sites. Restriction enzyme sites are indicated. SpletpCR2.1 TOPO: Analyze: Sequence: Plasmid Type: Bacterial Expression: Promotor: Plac: Cloning Method: TOPO Cloning: Size: 3900: 5' Sequencing 1 Primer: M13 reverse: 5' …

SpletDescription The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. … Splet2-amplify by PCR from the plasmid PCR TOPO with primer including restriction site you need (with more than 5nt between ends and restriction site) 3-migration of 5µl of the PCR product 4- if no...

SpletBasic Vector Information; Vector Name: pCR2.1-TOPO: Antibiotic Resistance: Ampicillin Length: 3931 bp: Type: I.M.A.G.E. Consortium Plasmids Source: Invitrogen (Life …

SpletDescription. Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. 3'-T overhangs for direct ligation of Taq-amplified PCR products ; Choice of T7 (pCR2.1-TOPO™) or T7 and SP6 (pCRII-TOPO™) promoters for in vitro RNA transcription and sequencing.Each vector also contains M13 forward and reverse primer … shanghai venice of the eastSplet19. jul. 2015 · 为了研究MORC家族ATP酶的功能特性，Invitrogen的pCR2.1 TOPO载体被用于基因克隆。 至该文献 为了研究人胚胎干细胞分化过程中p53的作用，采用了Invitrogen公司的pENTR1a，进行DNA克隆实验。 至该文献 为了研究先天免疫对李斯特菌和LPS的应答是通过TNF介导且TNF受iRhom2调控的TACE控制，采用了Invitrogen的pCR2.1-TOPO进行质粒 … shanghai vigovita biotech co. ltdSpletpCR-Blunt II-TOPO Parental vector for TOPO® cloning of blunt PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:I.M.A.G.E. Consortium Plasmids More Plasmid Sets No matches HomePlasmidsI.M.A.G.E. Consortium … shanghai victoria secret fashion show 2017SpletPlasmid pCR2.1_HBB_N from Dr. Marina Kleanthous's lab contains the insert Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) and is published in Int J Mol Sci. 2024 Sep 11;21(18). pii: ijms21186671. doi: 10.3390/ijms21186671. ... pCR2.1-TOPO (Search Vector Database) Backbone manufacturer. Invitrogen (Life Technologies ... shanghai victoria secret fashion show robeSpletThe sequence of pCR®2.1-TOPO has been compiled from information in sequence databases, published sequences, and other sources. Portions of this vector have not yet … polyester fabric good for winterSpletTrue, iam using Invitrogen's pCR2.1 TOPO TA Cloning vector having BamHI and HindIII sites. I usually send the purified plasmid for sequencing. Do you suggest me to amplify the goi with pcr primers ... shanghai victoriaSpletpCR2.1-TOPO. Parental bacterial expression vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Try SnapGene for Free. … shanghai vift machinery co. ltd